Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field. The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods. However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.
《蛋白质表达与纯化》是一本国际性期刊，为传播有关蛋白质表达、提取、纯化、表征和/或应用的新信息提供了一个论坛，使用传统的生物化学和/或现代分子生物学方法和方法，这些方法和方法在该领域具有广泛的兴趣。该杂志通常不发表涉及标准的、完善的方法的蛋白质表达和纯化的重复实例。但是，例外情况可能包括重要和/或难以表达和/或纯化蛋白质的研究和/或包括广泛蛋白质表征的研究，这些研究提供了新的、先前未发表的信息。
Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.09.006
Cloning, expression and characterization of an endo-acting bifunctional alginate lyase of marine bacterium Wenyingzhuangia fucanilytica.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.09.010
Purification and characterization of a new xylanase with excellent stability from Aspergillus flavus and its application in hydrolyzing pretreated corncobs.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.10.006
Expression and purification of recombinant G protein-coupled receptors: A review
来源期刊：Protein Expression and PurificationDOI：10.1016/j.pep.2019.105524
A two-step process for capture and purification of human basic fibroblast growth factor from E. coli homogenate: Yield versus endotoxin clearance.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.08.009
Gene dosage and coexpression with endoplasmic reticulum secretion-associated factors improved the secretory expression of α-galactosidase.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.08.004
An efficient constitutive expression system for Anti-CEACAM5 nanobody production in the yeast Pichia pastoris.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.11.001
Enhancement of the production of Bacillus naganoensis pullulanase in recombinant Bacillus subtilis by integrative expression.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.03.006
Characterization and expression in Pichia pastoris of a raw starch degrading glucoamylase (GA2) derived from Aspergillus flavus NSH9.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105462
Design, production and purification of a novel recombinant gonadotropin-releasing hormone associated peptide as a spawning inducing agent for fish.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105510
Recombinant expression, purification, and characterization of a cyclodextrinase from Massilia timonae.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.08.013
Application of crossflow ultrafiltration for scaling up the purification of a recombinant ferritin.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105451
Expression of recombinant tachyplesin I in Pichia pastoris.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.01.012
A fully automated three-step protein purification procedure for up to five samples using the NGC chromatography system.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.08.003
Expression, purification and characterisation of Trypanosoma congolense metacaspase 5 (TcoMCA5) - a potential drug target for animal African trypanosomiasis.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105465
Expression, purification, and characterization of the Degludec precursor DesB30.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.04.010
Cloning and expression of pullulanase from Bacillus subtilis BK07 and PY22 in Pichia pastoris.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.05.008
Expression, purification and molecular characterization of a novel transcription factor KcCBF3 from Kandelia candel.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.08.006
Targeted expression and purification of fluorine labelled cold shock protein B by using an auxotrophic strategy.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.02.006
Recombinant expression and purification of a functional bacterial metallo-chaperone PbrD-fusion construct as a potential biosorbent for Pb(II).
来源期刊：Protein Expression and PurificationDOI：10.1016/J.PEP.2019.02.008
Cloning, expression, and purification of the recombinant pro-apoptotic dominant-negative survivin T34A-C84A protein in Escherichia coli.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.04.003
Introducing a cost-effective method for purification of bioactive flagellin from several flagellated gram-negative bacteria.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.11.007
Efficient expression and secretion of endo-1,4-β-xylanase from Penicillium citrinum in non-conventional yeast Yarrowia lipolytica directed by the native and the preproLIP2 signal peptides.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.03.012
High-level heterologous expression of the transmembrane human sterol Δ8,Δ7-isomerase in Pichia pastoris.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105463
Screening and production of an affibody inhibiting the interaction of the PD-1/PD-L1 immune checkpoint.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105520
Cloning and high-level SUMO-mediated fusion expression of a serine protease inhibitor from Hyphantria cunea Drury that exhibits activity against papain.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.02.011
Development of a biochemical and biophysical suite for integral membrane protein targets: A review.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105545
Cloning, overexpression and purification of a novel two-domain protein of Staphylococcus aureus phage Phi11.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.10.005
Soluble expression of mature Rhizopus chinensis lipase in Escherichia coli and enhancement of its ester synthesis activity.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.06.003
Cloning and purification of an anti-thrombotic, chimeric Staphylokinase in Pichia pastoris.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.05.004
Cloning, characterization and expression analysis of glutathione S-transferase from the Antarctic yeast Rhodotorula mucilaginosa AN5.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105518
cDNA cloning, prokaryotic expression and functional analysis of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) in Pogostemon cablin.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105454
Review of lactose-driven auto-induction expression of isotope-labelled proteins.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.01.007
Molecular characterization of a highly efficient and thermostable phosphoribosyl anthranilate isomerase from Geobacillus thermopakistaniensis.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105523
Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli
来源期刊：Protein Expression and PurificationDOI：10.1016/j.pep.2018.08.001
Expression of 5T4 extracellular domain fusion protein and preparation of anti-5T4 monoclonal antibody with high affinity and internalization efficiency.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.07.003
pH conditioning is a crucial step in primary recovery - A case study for a recombinant Fab from E. coli.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105504
Schistosoma mansoni cathepsin D1: Biochemical and biophysical characterization of the recombinant enzyme expressed in HEK293T cells.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105532
Expression, purification and stabilization of human serotonin transporter from E. coli.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105479
Enzymatic characteristics of a recombinant protease (rPepD) from Aspergillus niger expressed in Pichia pastoris.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.06.002
Purification and characterization of the extracellular region of human receptor tyrosine kinase like orphan receptor 2 (ROR2).
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.02.015
Expression, purification, and in vitro characterization of kinase domain of NtGCN2 from tobacco.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105452
Enhancement of the stability of Mycobacterium tuberculosis recombinant antigen expressed in Escherichia coli using cell lysis additives.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105453
The effects of overexpression of cytoplasmic chaperones on secretory production of hirudin-PA in E. coli.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.01.011
A Scalable Platform for Producing Recombinant Nucleosomes with Codified Histone Methyltransferase Substrate Preferences.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105455
Expression, purification, and structural analysis of the full-length human integral membrane protein γ-sarcoglycan.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.105525
The adequate amount of sodium chloride in Protein A wash buffer for effective host cell protein clearance.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.02.016
Purification and characterization of native human elongation factor 2.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2019.02.005
Expression of Halo-hFGF18 and study of its effect on differentiation of ATDC5 cells.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.10.010
Expression, purification and biological activity of monomeric insulin precursors from methylotrophic yeasts.
来源期刊：Protein expression and purificationDOI：10.1016/j.pep.2018.08.002
