Nature Protocols aims to publish the protocols being used to answer outstanding biological and biomedical science research questions, including methods grounded in physics and chemistry that have a practical application to the study of biological problems. As the main audience for Nature Protocols articles is research scientists, we only publish protocols that have research applications; thus, we do not publish protocols where the main application is to make decisions that influence patient management and treatment. Specific techniques of interest include, but are not limited to, protocols relating to: Biochemistry; Cell biology; Cell culture; Chemical modification; Computational biology; Developmental biology; Epigenomics; Genetic analysis; Genetic modification; Genomics; Imaging; Immunology; Isolation, purification and separation; Lipidomics; Metabolomics; Microbiology; Model organisms; Nanotechnology; Neuroscience; Nucleic-acid based molecular biology; Pharmacology; Plant biology; Protein analysis; Proteomics; Spectroscopy; Structural biology; Synthetic chemistry; Tissue culture; Toxicology; Virology.
《自然协议》旨在出版用于回答突出的生物学和生物医学科学研究问题的协议，包括基于物理学和化学的方法，这些方法对生物学问题的研究具有实际应用。因为Nature Protocols文章的主要读者是研究科学家，所以我们只发表具有研究应用的协议;因此，在主要应用是做出影响患者管理和治疗的决定的情况下，我们不公布协议。感兴趣的具体技术包括但不限于涉及以下方面的协议：生物化学;细胞生物学;细胞培养;化学改性;计算生物学发育生物学;表观基因组学;遗传分析;基因改造;基因组学;成像;免疫学;分离、纯化、分离;脂质组学;代谢组学;微生物学;模式生物;纳米技术;神经科学;核酸分子生物学;药理学;植物生物学;蛋白质分析;蛋白质组学;光谱学;结构生物学合成化学;组织培养;毒理学;病毒学
Protocol Update for large-scale genome and gene function analysis with the PANTHER classification system (v.14.0)
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0128-8
Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0098-2
Generation of lung organoids from human pluripotent stem cells in vitro
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0104-8
Using DeepLabCut for 3D markerless pose estimation across species and behaviors
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0176-0
High-resolution 3D imaging of fixed and cleared organoids
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0160-8
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0164-4
Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0127-9
Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0113-7
A nanostructure platform for live-cell manipulation of membrane curvature
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0161-7
Fabrication and practical applications of molybdenum disulfide nanopores
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0131-0
Standardization of complex biologically derived spectrochemical datasets
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0150-x
Microfabricated blood vessels for modeling the vascular transport barrier
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0144-8
Induction of neoantigen-reactive T cells from healthy donors
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0170-6
Using the tube test to measure social hierarchy in mice
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0116-4
A practical guide to intelligent image-activated cell sorting
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0183-1
Bioluminescence resonance energy transfer–based imaging of protein–protein interactions in living cells
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0129-7
Isolating the human cochlea to generate bone powder for ancient DNA analysis
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0137-7
RNA sequencing and swarm intelligence–enhanced classification algorithm development for blood-based disease diagnostics using spliced blood platelet RNA
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0139-5
Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0187-x
High-throughput phenotyping with electronic medical record data using a common semi-supervised approach (PheCAP)
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0227-6
Diselenide–selenoester ligation for chemical protein synthesis
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0180-4
Derivation of enteric neuron lineages from human pluripotent stem cells
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0141-y
Design, execution, and analysis of CRISPR–Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0122-6
Wireless nanopore electrodes for analysis of single entities
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0171-5
Nongenetic optical neuromodulation with silicon-based materials
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0135-9
High-throughput multicolor optogenetics in microwell plates
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0178-y
Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0168-0
Multiplexed profiling of RNA and protein expression signatures in individual cells using flow or mass cytometry
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0120-8
Synthetic chemistry in water: applications to peptide synthesis and nitro-group reductions
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0130-1
In-situ observation of conformational dynamics and protein-ligand/substrate interactions in outer membrane proteins with DEER/PELDOR spectroscopy
来源期刊：Nature protocolsDOI：10.1038/s41596-019-0182-2
Pipelines for cross-species and genome-wide prediction of long noncoding RNA binding
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0115-5
Integrating hydrogen–deuterium exchange mass spectrometry with molecular dynamics simulations to probe lipid-modulated conformational changes in membrane proteins
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0219-6
Using enhanced number and brightness to measure protein oligomerization dynamics in live cells
来源期刊：Nature ProtocolsDOI：10.1038/s41596-018-0111-9
Facile 18F labeling of non-activated arenes via a spirocyclic iodonium(III) ylide method and its application in the synthesis of the mGluR5 PET radiopharmaceutical [18F]FPEB
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0149-3
Using Cre-recombinase-driven Polylox barcoding for in vivo fate mapping in mice
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0163-5
Self-assembly and characterization of 2D plasmene nanosheets
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0200-4
MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0223-x
Transgenesis in Hydra to characterize gene function and visualize cell behavior
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0173-3
Magnetically guided virus stamping for the targeted infection of single cells or groups of cells
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0221-z
Creating functional chromosome fusions in yeast with CRISPR–Cas9
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0192-0
Stable isotope labeling of metal/metal oxide nanomaterials for environmental and biological tracing
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0205-z
Regeneration of a bioengineered 3D integumentary organ system from iPS cells
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0124-z
One-step site-specific antibody fragment auto-conjugation using SNAP-tag technology
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0214-y
Bio-additive-based screening: toward evaluation of the biocompatibility of chemical reactions
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0190-2
Capturing 5ʹ and 3ʹ native ends of mRNAs concurrently with Akron sequencing
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0151-9
Genome-wide profiling of nucleosome position and chromatin accessibility in single cells using scMNase-seq
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0243-6
Author Correction: A practical guide to intelligent image-activated cell sorting
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0252-5
Publisher Correction: Live imaging of stem cells in the germarium of the Drosophila ovary using a reusable gas-permeable imaging chamber
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0134-x
A marker-independent lineage-tracing system to quantify clonal dynamics and stem cell functionality in cancer tissue
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0194-y
Author Correction: V2a interneuron differentiation from mouse and human pluripotent stem cells
来源期刊：Nature ProtocolsDOI：10.1038/s41596-019-0266-z
