The first volume of enzymatic methods was published in 1955. It has become the most popular and respected method series in the field of biochemistry. This set of books has a history of more than 50 years and has been published in more than 540 volumes. Most of the information content of this series of books with more than 540 volumes is closely related to current research. It is not a book that only introduces enzymology, but a series of books covering all branches of life science. It is a set of basic and necessary reference resources for researchers in the field of life science, including microbiology, biochemistry, cancer research, genetics, etc.nzymology methods is the series with the highest citation rate on ScienceDirect and the most downloaded books. At present, we can access the contents of enzymatic methods from 2000 to now, and the details are subject to the page display.
酶学方法(Methods In Enzymology)是一本由Academic Press Inc.出版的一本生物-生化研究方法学术刊物，主要报道生物-生化研究方法相关领域研究成果与实践。本刊已入选来源期刊，该刊创刊于1955年，出版周期Irregular。2023年发布的影响因子为0，CiteScore指数2.9，SJR指数0.133。本刊非开放获取期刊。酶学方法第一卷出版于1955年，成为生物化学领域最受欢迎、最受推崇的方法系列丛书。这套丛书已有50多年的历史，已出版540余卷。这套540余卷的丛书大部分信息内容与当前研究密切相关。它不是一本只介绍酶学的书籍，而是涵盖生命科学各个分支的系列丛书，是微生物学、生物化学、癌症研究、遗传学等生命科学领域研究人员的一套基本、必备的参考资源。酶学方法是ScienceDirect上引用率最高、下载书籍最多的系列丛书，目前可以查阅2000年至今的酶学方法内容，详情以页面展示为准。
Characterization of Internal Protein Dynamics and Conformational Entropy by NMR Relaxation.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.09.010
A pipeline for characterization of novel Cas9 orthologs.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.10.021
Reverse Micelle Encapsulation of Proteins for NMR Spectroscopy.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.08.032
Overhauser Dynamic Nuclear Polarization for the Study of Hydration Dynamics, Explained.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.09.024
Profiling DUBs and Ubl-specific proteases with activity-based probes
来源期刊：Methods in EnzymologyDOI：10.1016/bs.mie.2018.12.037
Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.08.030
Light-activation of Cre recombinase in zebrafish embryos through genetic code expansion.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.004
Differentiation of peptide isomers and epimers by radical-directed dissociation.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.020
Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.003
CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.10.026
TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.05.006
Performing anaerobic stopped-flow spectrophotometry inside of an anaerobic chamber.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.006
Cell-surface display of designer cellulosomes by Lactobacillus plantarum.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.011
Methods to study SUMO dynamics in yeast.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.026
Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.010
In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.028
Integrating computational and experimental methods for efficient biocatalytic synthesis of polyesters.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.07.040
Synthetic cell-like membrane interfaces for probing dynamic protein-lipid interactions.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.02.015
Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.016
Analysis of human cGAS activity and structure.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.012
An SPR-based analysis of cGAS substrate KD and steady-state KM values.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.023
Phosphodiester photo-tethers for the (multi-)cyclic conformational caging of oligonucleotides.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.019
Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.11.006
EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.017
Methods for determining the reduction potentials of flavin enzymes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.004
Metabolomics analysis of lipid metabolizing enzyme activity.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.06.027
Protein interactions of the inflammasome adapter ASC by solution NMR.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.07.008
In vitro assembly of thermostable Csm complex in CRISPR-Cas type III/A system.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.10.025
Protocols for the generation of caged guideRNAs for light-triggered RNA-targeting with SNAP-ADARs.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.004
Combinatorial control of gene function with wavelength-selective caged morpholinos.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.007
Optogenetic perturbation of the biochemical pathways that control cell behavior.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.020
Differentiation and classification of RNA motifs using small molecule-based pattern recognition.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.05.022
Analysis of cardiac troponin proteoforms by top-down mass spectrometry.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.07.029
Molecular recognition of HIV-1 RNAs with branched peptides.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.021
Reversible optogenetic control of protein function and localization.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.05.002
Investigations of two-component flavin-dependent monooxygenase systems.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.018
Quantification of IDO1 enzyme activity in normal and malignant tissues.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.07.006
Drop-in-well chamber for droplet interface bilayer with built-in electrodes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.012
Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.004
Reduction midpoint potentials of bifurcating electron transfer flavoproteins.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.017
Unraveling flavoenzyme reaction mechanisms using flavin analogues and linear free energy relationships.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.010
A guide for drug inducible genome editing with HIT systems.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.031
Synthesis of glycomonomers via biocatalytic methods.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.015
Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imaging.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.07.031
Applications of Dissolution-DNP for NMR Screening.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.08.016
An integrative protocol for the structure determination of the mouse ASC-PYD filament.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.033
A guide for drug inducible transcriptional activation with HIT systems.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.032
Single-cell assays using integrated continuous-flow microfluidics.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.014
Chemical probes for spatially resolved measurement of active enzymes in single cells.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.017
Nanokits for the electrochemical quantification of enzyme activity in single living cells.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.015
