Characterization of Internal Protein Dynamics and Conformational Entropy by NMR Relaxation.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.09.010
A pipeline for characterization of novel Cas9 orthologs.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.10.021
Reverse Micelle Encapsulation of Proteins for NMR Spectroscopy.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.08.032
Overhauser Dynamic Nuclear Polarization for the Study of Hydration Dynamics, Explained.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.09.024
Profiling DUBs and Ubl-specific proteases with activity-based probes
来源期刊：Methods in EnzymologyDOI：10.1016/bs.mie.2018.12.037
Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.08.030
Light-activation of Cre recombinase in zebrafish embryos through genetic code expansion.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.004
Differentiation of peptide isomers and epimers by radical-directed dissociation.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.020
Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.003
CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.10.026
TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.05.006
Performing anaerobic stopped-flow spectrophotometry inside of an anaerobic chamber.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.006
Cell-surface display of designer cellulosomes by Lactobacillus plantarum.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.011
Methods to study SUMO dynamics in yeast.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.026
Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.010
In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.028
Integrating computational and experimental methods for efficient biocatalytic synthesis of polyesters.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.07.040
Synthetic cell-like membrane interfaces for probing dynamic protein-lipid interactions.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.02.015
Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.016
Analysis of human cGAS activity and structure.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.012
An SPR-based analysis of cGAS substrate KD and steady-state KM values.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.023
Phosphodiester photo-tethers for the (multi-)cyclic conformational caging of oligonucleotides.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.019
Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.11.006
EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.12.017
Methods for determining the reduction potentials of flavin enzymes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.004
Metabolomics analysis of lipid metabolizing enzyme activity.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.06.027
Protein interactions of the inflammasome adapter ASC by solution NMR.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.07.008
In vitro assembly of thermostable Csm complex in CRISPR-Cas type III/A system.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.10.025
Protocols for the generation of caged guideRNAs for light-triggered RNA-targeting with SNAP-ADARs.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.004
Combinatorial control of gene function with wavelength-selective caged morpholinos.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.007
Optogenetic perturbation of the biochemical pathways that control cell behavior.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.020
Differentiation and classification of RNA motifs using small molecule-based pattern recognition.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.05.022
Analysis of cardiac troponin proteoforms by top-down mass spectrometry.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.07.029
Molecular recognition of HIV-1 RNAs with branched peptides.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.021
Reversible optogenetic control of protein function and localization.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.05.002
Investigations of two-component flavin-dependent monooxygenase systems.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.018
Quantification of IDO1 enzyme activity in normal and malignant tissues.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.07.006
Drop-in-well chamber for droplet interface bilayer with built-in electrodes.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.012
Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.004
Reduction midpoint potentials of bifurcating electron transfer flavoproteins.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.017
Unraveling flavoenzyme reaction mechanisms using flavin analogues and linear free energy relationships.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.03.010
A guide for drug inducible genome editing with HIT systems.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.031
Synthesis of glycomonomers via biocatalytic methods.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.015
Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imaging.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2019.07.031
Applications of Dissolution-DNP for NMR Screening.
来源期刊：Methods in enzymologyDOI：10.1016/bs.mie.2018.08.016
An integrative protocol for the structure determination of the mouse ASC-PYD filament.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.04.033
A guide for drug inducible transcriptional activation with HIT systems.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.02.032
Single-cell assays using integrated continuous-flow microfluidics.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.014
Chemical probes for spatially resolved measurement of active enzymes in single cells.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.017
Nanokits for the electrochemical quantification of enzyme activity in single living cells.
来源期刊：Methods in enzymologyDOI：10.1016/BS.MIE.2019.06.015
