For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.
五十多年来，细胞生物学方法一直帮助研究人员回答“我应该用什么方法来研究这个细胞生物学问题？”由该领域的领导者编辑，每个主题卷提供经过验证的，最先进的技术，以及相关的历史背景和理论，以帮助研究人员有效设计和有效实施实验方法。在其多年的出版中，《细胞生物学方法》建立了一个深入的生物学方法库，以研究模型发育的生物，细胞器和细胞系统，以及显微镜和其他分析方法的全面报道。
Serial-section electron microscopy using automated tape-collecting ultramicrotome (ATUM).
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.04.004
Content-aware image restoration for electron microscopy.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.05.001
Genome-wide analysis of chromatin accessibility using ATAC-seq.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2018.11.002
Kinetic and photonic techniques to study chemotactic signaling in sea urchin sperm.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.12.001
Measuring surface expression and endocytosis of GPCRs using whole-cell ELISA.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.09.014
ADPKD cell proliferation and Cl--dependent fluid secretion.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.06.001
Measuring agonist-induced ERK MAP kinase phosphorylation for G-protein-coupled receptors.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.09.015
In situ analysis of male meiosis in C. elegans.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.03.013
Understanding GPCR dimerization.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.005
Procuring animals and culturing of eggs and embryos.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.11.006
Methods for the experimental and computational analysis of gene regulatory networks in sea urchins.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2018.10.003
Solution NMR spectroscopy of GPCRs: Residue-specific labeling strategies with a focus on 13C-methyl methionine labeling of the atypical chemokine receptor ACKR3.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.09.004
Intravital visualization of the primary cilium, tubule flow, and innate immune cells in the kidney utilizing an abdominal window imaging approach.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.04.012
Analysis of sperm chemotaxis.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.12.002
The role of β-arrestins in G protein-coupled receptor heterologous desensitization: A brief story.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.004
CRISPR/Cas9-mediated genome editing in sea urchins.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2018.10.004
Expedited large-volume 3-D SEM workflows for comparative microanatomical imaging.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.03.012
Live-cell fluorescence imaging of echinoderm embryos.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.10.006
Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.04.009
Non-radioactive binding assay for bradykinin and angiotensin receptors.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.002
Analysis of ubiquitination and ligand-dependent trafficking of group I mGluRs.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.008
Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare events.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.03.014
My research career on (mainly) sea urchins.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.03.003
Detection of misfolded rhodopsin aggregates in cells by Förster resonance energy transfer.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.007
Assessing real-time signaling and agonist-induced CRHR1 internalization by optical methods.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.009
Techniques for analyzing gene expression using BAC-based reporter constructs.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.01.004
Label-free impedance-based whole cell assay to study GPCR pharmacology.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.003
Temnopleurus as an emerging echinoderm model.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.09.001
Genetic manipulation of PLB-985 cells and quantification of chemotaxis using the underagarose assay.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.09.002
A teaching laboratory on the activation of xenobiotic transporters at fertilization of sea urchins.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.11.013
Measurement of cytoplasmic and cilioplasmic calcium in a single living cell.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.03.015
Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.04.014
Luciferase reporter assay for unlocking ligand-mediated signaling of GPCRs.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.08.001
Methods for toxicology studies in echinoderm embryos and larvae.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.11.011
Whole mount in situ hybridization techniques for analysis of the spatial distribution of mRNAs in sea urchin embryos and early larvae.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.01.003
Analysis of neural activity with fluorescent protein biosensors.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.10.010
When sperm meets egg-Fifty years of surprises.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.03.001
Unlocking mechanisms of development through advances in tools.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.03.005
Measuring voltage and ion concentrations in live embryos.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.01.007
Expression of exogenous mRNAs to study gene function in echinoderm embryos.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2018.10.011
Multiplex cis-regulatory analysis.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.01.009
Trapping, tagging and tracking: Tools for the study of proteins during early development of the sea urchin.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2018.11.003
A personal history of the echinoderm genome sequencing.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.03.008
In vivo analysis of renal epithelial cells in zebrafish.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.04.016
Probing Ca2+ release mechanisms using sea urchin egg homogenates.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.10.007
Quantifying autophagic flux in kidney tissue using structured illumination microscopy.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.05.004
Analysis of immune response in the sea urchin larva.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2018.10.009
Methods for renal lineage tracing: In vivo and beyond.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.06.002
Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells.
来源期刊：Methods in cell biologyDOI：10.1016/bs.mcb.2019.04.017
Spatially mapping gene expression in sea urchin primary mesenchyme cells.
来源期刊：Methods in cell biologyDOI：10.1016/BS.MCB.2019.01.006
