BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies.Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers:Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questionsDescriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devicesSurveys of technical approaches related to broad fields of researchReviews discussing advancements in techniques and methods related to broad fields of researchLetters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
BioTechniques是一份同行评审、开放获取的期刊，致力于发表原创实验室方法、相关技术和软件工具以及方法导向的综述文章，这些文章受到专业生命科学家以及其他学科科学家的广泛关注（例如：化学、物理学、计算机科学、植物和农业科学以及气候科学）。自1983年以来，《生物技术》一直是方法相关研究的领先同行评审期刊。该杂志考虑：报告描述创新的新方法、平台和软件，对现有方法的实质性修改，或现有方法、技术和工具对新模型或科学问题的创新应用描述促进实验或数据分析的设计或性能的技术工具，如软件和简单的实验室设备调查与广泛研究领域相关的技术方法评论讨论与广泛研究领域相关的技术和方法的进步给编辑的信和专家意见，突出有关实验设计、方法或分析的有趣观察或警示故事。
Recent advances in photo-crosslinkable hydrogels for biomedical applications.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0083
Rapid quantification of cellular proliferation and migration using ImageJ.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0132
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0192
A fast and simple fluorometric method to detect cell death in 3D intestinal organoids.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0023
Removal of extra sequences with I-SceI in combination with CRISPR/Cas9 technique for precise gene editing in Drosophila.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0147
Highly efficient library preparation for Ion Torrent sequencing using Y-adapters.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0035
A method for visualizing fluorescence of flavonoid therapeutics in vivo in the model eukaryote Dictyostelium discoideum.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0084
ELIMU-MDx: a web-based, open-source platform for storage, management and analysis of diagnostic qPCR data.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0064
A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0184
Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0026
A rapid approach to profiling diverse fungal communities using the MinION™ nanopore sequencer.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0072
A modified gas-trapping method for high-throughput metabolic experiments in Drosophila melanogaster.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0062
A robust fractionation method for protein subcellular localization studies in Escherichia coli.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0135
Comparison of normalization methods for Hi-C data.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0105
Efficient and robust preparation of tyrosine phosphorylated intrinsically disordered proteins.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0033
Protein and RNA quantification of multiple genes in single cells.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0130
Rapid extraction of DNA suitable for NGS workflows from bacterial cultures using the PDQeX.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0006
Cell detachment rapidly induces changes in noncoding RNA expression in human mesenchymal stromal cells.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0038
Rapid identification of genome-edited mesenchymal stem cell colonies via Cas9.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0183
A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of mRNA.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0120
Idiosyncrasies of thermofluorimetric aptamer binding assays
来源期刊：BiotechniquesDOI：10.2144/btn-2018-0128
Erythrosin B: a versatile colorimetric and fluorescent vital dye for bacteria.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0066
Fast, versatile and quantitative annotation of complex images.
来源期刊：BioTechniquesDOI：10.2144/BTN-2019-0010
High-throughput Quant-iT PicoGreen assay using an automated liquid handling system.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0172
Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0032
Ultra-fast conductive media for RNA electrophoretic mobility shift assays
来源期刊：BiotechniquesDOI：10.2144/btn-2019-0111
A simple in silico approach to generate gene-expression profiles from subsets of cancer genomics data.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0179
Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier
来源期刊：BiotechniquesDOI：10.2144/btn-2019-0083
Methodology for the at-home collection of urine samples for prostate cancer detection.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0092
Selection of reference genes suitable for normalization of RT-qPCR data in glioma stem cells.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0098
High-throughput quantitative detection of basal autophagy and autophagic flux using image cytometry.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0044
Spectral recording of gene expression history by fluorescent timer protein.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0050
Assessment of cell yield among different devices for endovascular biopsy to harvest vascular endothelial cells.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0099
A customized program for the identification of conserved protein sequence motifs.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0039
Combining CRISPR/Cas9-mediated knockout with genetic complementation for in-depth mechanistic studies in human ES cells
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0115
Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0163
Higher percentage of horse serum in culture media blocks attachment of PC12 cells
来源期刊：BiotechniquesDOI：10.2144/btn-2019-0073
A simple and high-throughput method for xylem sap collection.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0046
Improved locus-specific unmethylated controls for MS-HRM analysis derived from 5-aza-2-deoxycytidine-treated DNA.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0161
Advancing Precision Medicine.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0163
Towards the development of automated fed-batch cell culture processes at microscale.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0063
A rapid cell-based assay for determining poloxamer quality in CHO suspension cell culture.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0070
Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions.
来源期刊：BioTechniquesDOI：10.2144/btn-2018-0134
Native mass spectrometry: a powerful tool for structural biology?
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0131
Single-cell analysis for drug development using convex lens-induced confinement imaging.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0067
Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0001
Affimers as anti-idiotypic affinity reagents for pharmacokinetic analysis of biotherapeutics.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0089
Integrative approaches to cancer research.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0095
Brief freezing steps lead to robust immunofluorescence in the Drosophila nervous system
来源期刊：BiotechniquesDOI：10.2144/btn-2018-0067
A cautionary tale of cross-contamination among plasmids from commercial suppliers.
来源期刊：BioTechniquesDOI：10.2144/btn-2019-0018
