The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery.The methods may include, but not limited to, the study of:Viral components and morphology-Virus isolation, propagation and development of viral vectors-Viral pathogenesis, oncogenesis, vaccines and antivirals-Virus replication, host-pathogen interactions and responses-Virus transmission, prevention, control and treatment-Viral metagenomics and virome-Virus ecology, adaption and evolution-Applied virology such as nanotechnology-Viral diagnosis with novelty and comprehensive evaluation.We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.
《病毒学方法杂志》专注于原创的、高质量的研究论文，这些论文描述了新的、经过全面测试的方法，这些方法增强了人类、动物、植物、细菌或环境病毒学和朊病毒的研究和发现。这些方法可能包括但不限于以下研究：病毒成分和形态学-病毒分离、增殖和病毒载体的开发-病毒发病机理、肿瘤发生、疫苗和抗病毒药物-病毒复制、宿主-病原体相互作用和反应-病毒传播、预防、控制和治疗-病毒宏基因组学和病毒学-病毒生态学、适应和进化-应用病毒学，如纳米技术-具有新奇和综合评价的病毒诊断。我们寻求文章、系统综述、荟萃分析和实验室方案，这些方案包括全面的技术细节，并提供了针对当前最佳实践、国际标准或质量保证计划的验证，并推进了病毒学知识，从而改善了医疗
Critical issues in application of molecular methods to environmental virology.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.01.008
Development of a Real-Time Loop-Mediated Isothermal Amplification (LAMP) Assay and Visual LAMP Assay for Detection of African Swine Fever Virus (ASFV).
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113775
Clinical evaluation of multiplex RT-PCR assays for the detection of influenza A/B and respiratory syncytial virus using a high throughput system.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.03.015
In-field capable loop-mediated isothermal amplification detection of Turnip yellows virus in plants and its principal aphid vector Myzus persicae.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.12.014
Screening of novel drugs for inhibiting hepatitis E virus replication.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.04.017
Validation of serological and molecular methods for diagnosis of zika virus infections.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.10.011
Serotyping of foot-and-mouth disease virus using oxford nanopore sequencing.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.10.020
Added value of IgA antibodies against Zika virus non-structural protein 1 in the diagnosis of acute Zika virus infections.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.02.005
Performance of Bio-Rad HIV-1/2 Confirmatory Assay in HIV-1, HIV-2 and HIV-1/2 dually reactive patients - comparison with INNO-LIA and immunocomb discriminatory assays.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.03.005
Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.04.004
Common vole (Microtus arvalis) and bank vole (Myodes glareolus) derived permanent cell lines differ in their susceptibility and replication kinetics of animal and zoonotic viruses.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113729
Recombinant DENV 2 NS5: An effective antigen for diagnosis of DENV infection.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.12.005
Rapid detection of Cucumber green mottle mosaic virus in watermelon through a recombinase polymerase amplification assay.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.05.008
Rapid and sensitive detection of goose parvovirus and duck-origin novel goose parvovirus by recombinase polymerase amplification combined with a vertical flow visualization strip.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.01.010
Development and evaluation of a one-step reverse transcription loop-mediated isothermal amplification for detection of Citrus leaf blotch virus.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.05.009
Establishment and application of a multiplex RT-PCR to differentiate wild-type and vaccine strains of porcine epidemic diarrhea virus.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113684
Comparative analysis of the performance of residual host cell DNA assays for viral vaccines produced in Vero cells.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.01.001
Deep sequencing prompts the modification of a real-time RT-PCR for the serotype-specific detection of polioviruses
来源期刊：Journal of Virological MethodsDOI：10.1016/j.jviromet.2018.11.007
Methods for successful inactivation of Rift Valley fever virus in infected mosquitoes.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113794
Development and pre-validation of a quantitative multi-dose serological assay for potency testing of inactivated rabies vaccines for human use.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.10.003
Construction of replication-competent oncolytic retroviral vectors expressing R peptide-truncated 10A1 envelope glycoprotein.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.03.008
A New Solid Matrix for Preservation of Viral Nucleic Acid from Clinical Specimens at Ambient Temperature.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113732
Development of a triplex real-time RT-PCR assay for detection and differentiation of three US genotypes of porcine hemagglutinating encephalomyelitis virus
来源期刊：Journal of Virological MethodsDOI：10.1016/j.jviromet.2019.04.008
Development and evaluation of an indirect ELISA based on recombinant nonstructural protein 3A to detect antibodies to duck hepatitis A virus type 1.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.03.012
TaqMan real-time and conventional nested PCR tests specific to Yellow head virus genotype 7 (YHV7) identified in Giant tiger shrimp in Australia.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113689
Comparison of nucleic acid extraction methods for next-generation sequencing of avian influenza A virus from ferret respiratory samples.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.04.014
One-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.04.005
Application of high-resolution melting curve analysis for identification of Muscovy duck parvovirus and goose parvovirus.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.12.018
Duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemum.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.12.007
Rapid detection of two emerging viruses associated with necrotic symptoms in Areca catechu L. by reverse transcription loop-mediated isothermal amplification (RT-LAMP).
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113795
Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y O and N types in potato.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.02.006
Predicting whole genome sequencing success for archived avian influenza virus (Orthomyxoviridae) samples using real-time and droplet PCRs.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113777
A universal random DNA amplification and labeling strategy for microarray to detect multiple pathogens of aquatic animals.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113761
Development of a colorimetric loop-mediated isothermal amplification assay for rapid and specific detection of Aves polyomavirus 1 from psittacine birds.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113687
Determination of the optimal method for the concentration and purification of 146S particles for foot-and-mouth disease vaccine production.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.04.009
Development of an enzyme-linked immunosorbent assay for Getah virus infection in horses using recombinant E2 protein as an antigen.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113681
A New Inactivation Method to Facilitate Cryo-EM of Enveloped, RNA Viruses Requiring High Containment: A Case Study using Venezuelan Equine Encephalitis Virus (VEEV).
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113792
Rapid identification of the Raccoon Rabies Virus Variant using a Real-time Reverse-Transcriptase Polymerase Chain Reaction.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113713
Simultaneous detection of three common potyviruses infecting cucurbits by Multiplex reverse transcription polymerase chain reaction assay.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113725
A sensitive nested multiplex RT-PCR assay for the simultaneous detection of three common viruses infecting pear plants.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.10.021
Development of an in-house ELISA to detect anti-HPV16-L1 antibodies in serum and dried blood spots.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2018.10.007
Generation of PK-15 cell lines highly permissive to porcine circovirus 2 infection by transposon-mediated interferon-gamma gene transfer.
来源期刊：Journal of virological methodsDOI：10.1016/j.jviromet.2019.113682
