Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics.Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.
《植物方法》是一本面向植物研究界的开放式、同行评审的在线期刊，涵盖了植物科学技术创新的各个方面。毫无疑问，我们已经进入了植物生物学令人兴奋的新时代。拟南芥基因组序列的完成，以及其他植物基因组学项目的快速进展，为植物科学所有领域的进步提供了无与伦比的机会。然而，如果我们要了解基因组中每一个基因的功能，以及各个部分是如何共同作用形成整个生物体的，那么巨大的挑战就摆在我们面前。实现这些目标需要前所未有的合作努力，将高通量、全系统技术与更有针对性的方法相结合，这些方法整合了细胞生物学、生物化学和分子遗传学等传统学科。技术创新可能是任何科学学科进步的最重要催化剂。工厂方法的目标是鼓励开发和采用新的和改进的技术和研究工具，并在适当的情况下，促进方法的一致性，以更好地整合来自不同实验室的数据。
PGA: a software package for rapid, accurate, and flexible batch annotation of plastomes
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0435-7
Measuring crops in 3D: using geometry for plant phenotyping
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0490-0
Accuracy assessment of plant height using an unmanned aerial vehicle for quantitative genomic analysis in bread wheat
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0419-7
Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0430-z
Combining gene expression programming and genetic algorithm as a powerful hybrid modeling approach for pear rootstocks tissue culture media formulation
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0520-y
DeepSeedling: deep convolutional network and Kalman filter for plant seedling detection and counting in the field
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0528-3
Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0411-2
Construction of a large-scale semi-field facility to study genotypic differences in deep root growth and resources acquisition
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0409-9
Flowers, leaves or both? How to obtain suitable images for automated plant identification
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0462-4
Partial and full root-zone drought stresses account for differentiate root-sourced signal and yield formation in primitive wheat
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0461-5
Machine learning driven non-invasive approach of water content estimation in living plant leaves using terahertz waves
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0522-9
Use of X-ray micro computed tomography imaging to analyze the morphology of wheat grain through its development
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0468-y
Differential contributions of NO3−/NH4+ to nitrogen use in response to a variable inorganic nitrogen supply in plantlets of two Brassicaceae species in vitro
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0473-1
Discrimination between regional biotypes of Impatiens glandulifera using a simple MALDI-TOF MS-based method for use with seeds
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0412-1
Spectroscopic determination of leaf chlorophyll content and color for genetic selection on Sassafras tzumu
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0458-0
Comparative Subsequence Sets Analysis (CoSSA) is a robust approach to identify haplotype specific SNPs; mapping and pedigree analysis of a potato wart disease resistance gene Sen3
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0445-5
Assessing plant performance in the Enviratron
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0504-y
Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0455-3
Phenotyping viral infection in sweetpotato using a high-throughput chlorophyll fluorescence and thermal imaging platform
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0501-1
An efficient RGB-UAV-based platform for field almond tree phenotyping: 3-D architecture and flowering traits
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0547-0
Centromere histone H3- and phospholipase-mediated haploid induction in plants
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0429-5
A customizable method to characterize Arabidopsis thaliana transpiration under drought conditions
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0474-0
Instantaneous and non-destructive relative water content estimation from deep learning applied to resonant ultrasonic spectra of plant leaves
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0511-z
Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0404-1
A low-cost aeroponic phenotyping system for storage root development: unravelling the below-ground secrets of cassava (Manihot esculenta)
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0517-6
Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0459-z
A novel strategy to enhance terpenoids production using cambial meristematic cells of Tripterygium wilfordii Hook. f.
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0513-x
Sensory assessment of Cercospora beticola sporulation for phenotyping the partial disease resistance of sugar beet genotypes
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0521-x
3DCellAtlas Meristem: a tool for the global cellular annotation of shoot apical meristems
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0413-0
A low-cost and open-source platform for automated imaging
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0392-1
Alternative methods of estimating the water potential at turgor loss point in Acer genotypes
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0410-3
Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0451-7
Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0548-z
A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0536-3
A simple technique for assessing the cuticular diffusion of humic acid biostimulants
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0469-x
Hemoglobin as a probe for estimation of nitric oxide emission from plant tissues
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0425-9
Image analysis-based recognition and quantification of grain number per panicle in rice
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0510-0
Evaluation of grain yield based on digital images of rice canopy
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0416-x
Expanding the bioluminescent reporter toolkit for plant science with NanoLUC
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0454-4
Comparing time series transcriptome data between plants using a network module finding algorithm
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0440-x
Virus-induced gene silencing (VIGS) in Cannabis sativa L.
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0542-5
A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0471-3
High-throughput long paired-end sequencing of a Fosmid library by PacBio
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0525-6
Heterologous overexpression, purification and functional analysis of plant cellulose synthase from green bamboo
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0466-0
Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0541-6
An in vitro protocol for rapidly assessing the effects of antimicrobial compounds on the unculturable bacterial plant pathogen, Candidatus Liberibacter asiaticus
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0465-1
An improved method of constructing degradome library suitable for sequencing using Illumina platform
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0524-7
Correction to: Contour analysis for interpretable leaf shape category discovery
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0516-7
A high-throughput amplicon-based method for estimating outcrossing rates
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0433-9
Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes
来源期刊：Plant MethodsDOI：10.1186/s13007-019-0491-z
